KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF A 3-ARYL-3-HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE
摘要
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)-N,N-dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
申请公布号
WO2010025238(A3)
申请公布日期
2010.07.29
申请号
WO2009US55175
申请日期
2009.08.27
申请人
CODEXIS, INC.;SAVILE, CHRISTOPHER;GRUBER, JOHN, M.;MUNDORFF, EMILY;HUISMAN, GJALT, W.;COLLIER, STEVEN, J.
发明人
SAVILE, CHRISTOPHER;GRUBER, JOHN, M.;MUNDORFF, EMILY;HUISMAN, GJALT, W.;COLLIER, STEVEN, J.