| 摘要 |
PROBLEM TO BE SOLVED: To provide an improved method for nucleic acid analysis in relation to the fields of molecular biology and nucleic acid chemistry. SOLUTION: The method includes: (a) providing an oligonucleotide which is complementary or partially complementary to the target nucleic acid molecule 100 labeled with at least one light emitting moiety; (b) providing at least one soluble light emission modifier 108 that modifies light emission from the labeled oligonucleotide to a greater extent than from a labeled fragment of the oligonucleotide; (c) amplifying the nucleic acid in the sample in the presence of the labeled oligonucleotide and the soluble light emission modifier in an amplification reaction that comprises the selected condition such that the labeled oligonucleotide, hybridized with the target nucleic acid or the amplicon of the target nucleic acid, is cleaved to produce at least one labeled oligonucleotide fragment; and (d) detecting light emission from at least the labeled oligonucleotide fragment during (c), and thereby detecting the target nucleic acid. COPYRIGHT: (C)2010,JPO&INPIT
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