PCR METHOD

摘要

PROBLEM TO BE SOLVED: To provide a method for balancing multiplexed PCR methods.

SOLUTION: The method comprises two or more sequential temporal PCR stages used to effectively separate two or more PCR reactions in a single test tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during first and second amplification stages. Also provided are rapid RT-PCR methods that diagnose malignant esophageal adenocarcinoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.

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