发明名称 |
REAL-TIME, SINGLE-STEP BIOASSAY USING NANOPLASMONIC RESONATOR WITH ULTRA-HIGH SENSITIVITY |
摘要 |
A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes. |
申请公布号 |
EP2210072(A2) |
申请公布日期 |
2010.07.28 |
申请号 |
EP20080871173 |
申请日期 |
2008.11.03 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
CHEN, Fanqing, Frank;ELLMAN, Jonathan, A.;ZHANG, Xiang |
分类号 |
C12Q1/37;B82Y15/00;B82Y20/00;G01N21/65 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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