ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THE SAME

摘要

A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before.
(a) a protein having an amino acid sequence represented by SEQ ID NO. 23
(b) a protein having an amino acid sequence represented by SEQ ID NO. 25
(X) a primer that contains, in a 3' end portion, a sequence (Ac') that hybridizes to a sequence (A) of a 3' end portion of the target nucleic acid sequence and also contains, on a 5' side of the sequence (Ac'), a sequence (B') that hybridizes to a complementary sequence (Bc) to a sequence (B) present on a 5' side with respect to the sequence (A) in the target nucleic acid sequence