发明名称 |
Method for producing secreted proteins |
摘要 |
<p>The present invention discloses a method for producing a secreted biologically active mature ±-galactosidase A that contains ±2,6-sialic acid residues, comprising culturing an engineered mammalian cell that expresses ±-galactoside ±2,6-sialyltransferase such that the cell is capable of peptide sialylation, wherein the cell further comprises a nucleotide sequence that regulates gene expression operatively associated with an ±-galactosidase A gene sequence under conditions in which the ±-galactosidase A is sufficiently overexpressed by the engineered mammalian cell so as to be selectively secreted into the mammalian cell culture as a biologically active mature ±-galactosidase A that contains ±2,6-sialic acid residues; and isolating biologically active mature ±-galactosidase A that contains ±2,6-sialic acid residues from the mammalian cell culture.</p> |
申请公布号 |
EP2210947(A2) |
申请公布日期 |
2010.07.28 |
申请号 |
EP20100157183 |
申请日期 |
1993.11.30 |
申请人 |
MOUNT SINAI SCHOOL OF MEDICINE OF NEW YORK UNIVERSITY |
发明人 |
DESNICK, ROBERT J.;BISHOP, DAVID F.;IOANNOU, YIANNIS A. |
分类号 |
C12N15/09;C12N15/56;A61K35/18;A61K38/00;C07K14/31;C12N9/10;C12N9/24;C12N9/40;C12N15/54;C12N15/62;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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