VEGF BINDING V9 NANOANTIBODY AND METHOD OF OBTAINING SAID ANTIBODY, V9-CODING NUCLEOTIDE SEQUENCE AND VECTOR CONTAINING SAID SEQUENCE, ENDOTHELIAL CELL PROLIFERATION INHIBITION METHOD

摘要

FIELD: chemistry. ^ SUBSTANCE: invention relates to immunology and biotechnology. The invention describes a nucleotide sequence which codes the V9 nanoantibody and its expression vector with extra epitope(s) on the C-end for detection and extraction and a signal peptide on the N-end. The invention discloses a method of obtaining the V9 nanoantibody, a method of inhibiting proliferation of endothelial cells using the V9 nanoantibody, as well as use of the V9 nanoantibody for qualitative and quantitative determination of VEGF in a sample. Use of the invention provides high-affinity neutralising monovalent single-strand nanoantibodies which are more resistant to external factors (temperature, pH) and cheaper to produce compared to conventional VEGF antibodies, which can be useful in medicine for treating and diagnosing diseases associated with regulation of the activity of the vascular endothelial growth factor (VEGF). ^ EFFECT: invention discloses a monomer single-strand V9 nanoantibody which can bind and inhibit the human vascular endothelial growth factor. ^ 7 cl, 7 dwg, 7 ex