PCR PRIMER PAIR DETECTING PEANUT CLUMP VIRUS AND METHOD FOR DETECTING PEANUT CLUMP VIRUS USING THE SAME

摘要

PURPOSE: A PCR primer pair for detecting peanut clump virus(PCV) and a method for detecting PCV using the same are provided to quickly and accurately detect PCV from unknown sample. CONSTITUTION: A PCR primer pair for detecting peanut clump virus(PCV) comprises a forward primer selected from sequence numbers 1-10 and a reverse primer selected from sequence numbers 11 to 18. The PCR primer pair for detecting PCV comprises: a primer pair of sequence numbers 1 and 17, sequence numbers 4 and 14, sequence numbers 4 and 16, sequence numbers 5 and 13, sequence numbers 6 and 15, or sequence numbers 9 and 13. A method for detecting PCV from a sample comprises: a step of isolating RNA from the sample; a step of performing RT-PCR; and a step of separating PCR product by size.