发明名称 METHODS AND KITS TO GENERATE miRNA- AND small RNA-EXPRESSING VECTORS, AND ITS APPLICATION TO DEVELOP LENTIVIRAL EXPRESSION LIBRARIES
摘要 The invention relates to novel expression systems for microRNAs (miRNAs) based on the expression of the sense and antisense strand from convergent promoters. The invention also provides a method for the direct cloning of small RNAs present in an RNA preparation by the direct ligation of the small RNAs present within the preparation to vectors. Interestingly, if the vectors contain convergent promoter regions, the vectors isolated according to the method of the invention can be used for the direct expression of the small RNA without the need of using the DNA encoding for the small RNA precursor molecule.
申请公布号 WO2010081842(A2) 申请公布日期 2010.07.22
申请号 WO2010EP50370 申请日期 2010.01.14
申请人 NEWBIOTECHNIC, S.A.;CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;PINTOR TORO, JOSE ANTONIO;MORENO MATEOS, MIGUEL ANGEL;VALLE ROSADO, IVAN 发明人 PINTOR TORO, JOSE ANTONIO;MORENO MATEOS, MIGUEL ANGEL;VALLE ROSADO, IVAN
分类号 C12N15/09;C12N15/10;C12N15/11 主分类号 C12N15/09
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