| 摘要 |
<P>PROBLEM TO BE SOLVED: To construct a screening method for search of a substance specifically inhibiting the activity of a tRNA-dependent amidotransferase (GatCAB), to simply measure the deterioration of the GatCAB activity in which the construction of an enzymic reaction system such as substrate preparation is difficult, and to efficiently screen an enzyme-specific inhibitor expected as a medicament. Ž<P>SOLUTION: A coliform bacillus co-transformant of a plasmid starting transcription with a coliform bacillus RNA polymerase, and strictly controlling expression of a non-discriminating glutamyl-tRNA synthetase (ndGluRS) gene, and a plasmid permanently expressing GatCAB gene is constructed. The coliform bacillus is used as a test bacillus, and the deterioration of the GatCAB activity can readily be detected by measuring the growth of the coliform bacillus without preparing a substrate necessary for enzyme activity measurement and an enzyme protein. An inhibitor specific for the GatCAB can simply be searched for by screening a substance inhibiting the growth of the test bacillus without affecting a non-transformant. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
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