摘要 |
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products. |
申请人 |
WESTEND ASSET CLEARINGHOUSE COMPANY, LLC;JACOBSON, JOSEPH;CHURCH, GEORGE;CHU, LARRY, LI-YANG |
发明人 |
JACOBSON, JOSEPH;CHURCH, GEORGE;CHU, LARRY, LI-YANG |