PARALLEL SCREENING SUPERCRITICAL FLUID CHROMATOGRAPHY

摘要

The invention provides an apparatus for supercritical fluid chromatography. The apparatus comprises a binary pump; an autosampler; a sampling valve; a first and second port switching valve; a first and second manifold; two or more channels, each having a check valve assembly, a separation column and one or more detectors operatively connected thereon; and a backpressure regulator. The apparatus also includes computer software and hardware to control distribution of fluid through the apparatus, including switching between a multi-channel mode or a single channel ode; 2) analyze data collected by the one or more detectors; and 3) optimize separation of analytes by controlling solvent combinations, concentration gradients, pressure and temperature. The apparatus excludes additional backpressure regulators or pumps on individual channels. Also provided is a method of screening a sample, using supercritical chromatography, using the above apparatus, where multiple samples can be screened simultaneously with parallel processing.