PRIMER COMBINATION FOR DIAGNOSING RASPBERRY RINGSPOT VIRUS AND USES THEREOF

摘要

PURPOSE: A primer combination for raspberry ringspot virus(RpRSV) and a method for diagnosing RpRSV are provided to diagnose with high detection limit. CONSTITUTION: A primer combination for diagnosing RpRSV comprises forward primer and reverse primer. The forward primer is selected from base sequences of sequence numbers 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 19. The reverse primer is selected from base sequences of sequence numbers 23, 24, 25, 26, 28, 31, 32, 33, 34, 35, 36, 39, and 41. The primer combination for diagnosing RpRSV comprises a primer of sequence numbers 12 and 28, sequence number 11 and 28, or sequence numbers 8 and 31. A method for specifically diagnosing RpRSV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target seqeuence by RT-PCR; and a step of detecting the PCR product.