PRIMER COMBINATION FOR DIAGNOSING CHERRY LEAF ROLL VIRUS AND USES THEREOF

摘要

PURPOSE: A primer set for diagnosing cherry leaf roll virus(CLRV) and a method for diagnosing CLRV using the same are provided to test CVRV from a plant in immigration quarantine and clinical diagnosis. CONSTITUTION: A primer set for diagnosing CLRV comprises a forward primer and reverse primer. The forward primer is selected from sequence numbers 1, 2, 5, 6, 7, 8, 9 and 10. The reverse primer is selected from sequence numbers 14, 15, 16, 17, 18, 19, 20, 21 and 22. A kit for diagnosing CLRV comprises the primer set and DNA fragment having a base sequence of sequence number 24. A method for diagnosing CLRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product. The plant is cherry, berries, walnut, bean, plum, or pea.