NON-CONTACT METHODS OF DETECTING MOLECULAR COLONIES, SETS OF REAGENTS AND DEVICE FOR REALISING SAID METHODS

摘要

FIELD: chemistry. ^ SUBSTANCE: invention relates to methods of primer-dependent propagation of nucleic acids in immobilised media, particularly to methods of detecting products of such propagation. The invention discloses an improved method of identifying changes in a nucleotide sequence and determining the number of molecules of nucleic acids as separate molecules and contained in cells or in tissue fragments. The invention enables detection of molecular colonies of nucleic acids through fluorescence of at least growth of colonies in an immobilised medium (in real time) without an additional hybridisation step and without unsealing the chamber containing the medium. If the colony is formed by more than one target molecule, the invention enables to determine the number of target molecules from which the given colony originates. This in turn enables determination of spatial location and local concentration of RNA or DNA molecules of the given type in cells or in tissue fragments contained in the gel at the polymerisation step. ^ EFFECT: invention can be widely used as a diagnosis method and scientific research instrument. ^ 55 cl, 11 dwg, 12 ex