AN ANTI-PHYTOPHTHORA GENE CLUSTER SPECIFICALLY INHIBITING THE GROWTH OF PHYTOPHTHORA SPP. AND THE PREVENTION METHOD OF PHYTOPHTHORA USING THEREOF

摘要

PURPOSE: An antibacterial gene which is specific to phytophthora and a method for preventing plant disease using the same are provided to suppress growth of Phytophthora sp. strain. CONSTITUTION: A gene EnAP95(KACC 95090P) isolated from Enterobacter sp. YNB54 has a base sequence of sequence number 1. The gene EnAP95 is a Sal I fragment which is obtained by: selecting and analyzing E.coli clone which specifically suppresses Phytophthora capsici from a gene bank randomly produced by inserting Enterobacter sp. YNB54 chromosome DNA fragment to pBR322; isolating 20kb of fragment in which anti-phyrophthora gene is inserted; preparing mutant in which bacteriophage Mu is inserted; and selecting mutant of which antagonism to various phytophthora is weakened, lost, or strengthened.