摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for using a protein complex including a "ble" fusion protein having a specific bonding characteristic. Ž<P>SOLUTION: The protein complex can be bonded reversibly to an antibiotic from a bleomycin family, and the characteristic is utilized for various immobilization methods. The complex is used as a marker for protein expression and/or folding, or as a marker for affinity tagging. The invention also provides a probe including an array of an immobilized antibiotic from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which includes an antibiotic of the bleomycin family as an analyte capture moiety. Further, a method is provided, for generating a soluble form of an insoluble protein by expressing the protein as a "ble" fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the "ble" protein is expressed as a fusion protein in a cell into which a labelled antibiotic of the bleomycin family is introduced, thereby allowing identification of the cellular localization of the protein. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
|