发明名称 |
RECOVERY AND PURIFICATION OF B-PHYCOERYTHRIN PRODUCED BY PORPHYRIDIUM CRUENTUM USING TWO-AQUEOUS-PHASE SYSTEMS AND ISOELECTRIC PRECIPITATION |
摘要 |
This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid/liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm/Abs 280 nm).
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申请公布号 |
US2010178674(A1) |
申请公布日期 |
2010.07.15 |
申请号 |
US20070440458 |
申请日期 |
2007.08.16 |
申请人 |
INSTITUTIO TENOLOGICO Y DE ESTUDIOS SUPERIORES DEMONTERREY |
发明人 |
RITO PALOMARES MARCO ANTONIO;BENAVIDES LOZANO JORGE ALEJANDRO;HERNANDEZ MIRELES TANHIA DENYS |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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