METHODS AND MATERIALS FOR DETERMINING ISOELECTRIC POINT

摘要

Methods for determining an isoelectric point of an antibody may comprise identifying ammo acid residues in the sequence of the protein that are exposed on the surface of the protein, assigning a pKa value to the surface exposed amino acid residues, and calculating the isoelectric point of the protein from the pKa values assigned The isoelectric points may be used in methods for selecting and utilizing a buffer for purification of a protein, preparing a protein formulation, purifying a protein and/or stabilizing a protein in solution.