PRODUCITON OF NOVEL CHLOROGENIC ACID GLYCOSIDES USING GLUCANSUCRASE

摘要

PURPOSE: A method for manufacturing chlorogenic acid glycoside is provided to enhance solubility and preference and to use in foods, cosmetics, and medicines. CONSTITUTION: A chlorogenic acid glycoside is prepared using sugar and glucansucrase. The glucansucrase is obtained from Leuconostoc mesenteroides B-512FMCM. The enzyme reaction is performed by adding glucansucrase in a solution containing 0.1-1M of sugar and reacting at 28°C for 12 hours. A method for producing the chlorogenic acid glycoside comprises a step of isolating glycoside using non-polar solvent. The non-solvent is butanol.