发明名称 |
METHOD FOR PRODUCING PREPARATION OF RECOMBINANT AAV VIRION SUBSTANTIALLY FREE OF EMPTY CAPSID |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide a new method for removing AAV empty capsids from AAV vector particle stock or decreasing the number of AAV empty capsids, to improve production capacity. <P>SOLUTION: An efficient and commercially achievable method for preparing rAAV virion stock decreased in empty capsid amount is developed. It is found out that the empty capsids can be separated from the rAAV virion ("AAV vector particle") containing genetic materials by using column chromatography techniques. A method for separating empty capsids from a mixture of AAV vector and AAV empty capsids is provided. The method uses column chromatography techniques and provides for commercially achievable levels of recombinant AAV virions. <P>COPYRIGHT: (C)2010,JPO&INPIT |
申请公布号 |
JP2010155868(A) |
申请公布日期 |
2010.07.15 |
申请号 |
JP20100089074 |
申请日期 |
2010.04.07 |
申请人 |
GENZYME CORP |
发明人 |
QU GUANG;WRIGHT JOHN FRASER |
分类号 |
C07K14/075;C12N;C12N7/02;C12N15/09;C12N15/86;C12N15/864 |
主分类号 |
C07K14/075 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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