| 摘要 |
The present disclosure relates generally to methods and materials for detecting light absorbed or released from a labeling material. In particular, the present disclosure provides a detection architecture and devices employing the detection architecture for real-time subtraction of a background light from a light absorbed or released from a labeling material. Devices employing the detection architecture of the present disclosure may be used in methods for detecting a light absorbed or released from a labeling material by contacting a labeling material with a light source, wherein the fluorescent label absorbs or releases a first light; detecting the first light after the light source is enabled with a detector, wherein the detector produces a first signal from the first light; turning on a first switch at a first time for a fixed interval to receive the first signal from the first light; detecting a background noise with a detector after the light source is disabled, wherein the detector produces a second signal from the background noise; turning on a second switch at a second time for a fixed interval to receive the second signal from the second light; feeding the first signal and the second signal into a differential amplifier, wherein the differential amplifier subtracts the second signal from the first signal and produces an output signal; and measuring the output signal emitted from the differential amplifier.
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