| 摘要 |
Fresh tissue slices can be cut from tissue specimens embedded in a low gel point agarose by the use of a vibrating, sliding-blade microtome. The microtome has a blade which vibrates in a generally horizontal direction and which is supported for movement in a generally diagonal, sliding direction with respect to the tissue specimen. The compound vibrating and sliding motion of the cutting blade of the microtome is very effective in cutting fresh tissue slices that have a slice viability, measured as a ratio of live to dead cells, which is much better than that which has been obtained using conventional vibrating microtomes.
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