VARIANT LOXP SEQUENCES AND APPLICATION OF THE SAME

摘要

Variant loxP sequences having the characteristics (a) to (c) as specified below whereby gene insertion or gene substitution can be effected at a high efficiency in higher eucaryotic cells such as animal cells: (a) having a sequence derived from the following wild type loxP sequence originating in a coliphage P1 by substitution of at least one of the bases at the 2-, 3-, 4-and 5-positions (i.e., T, G, T and A, respectively) and at least one of the bases at the 6- and 7-positions (i.e., T and G, respectively) in the central eight bases (the spacer region) each by a different base optionally with substitution of base(s) in the regions other than the spacer region by arbitrary base(s): 12345678 5'-ATAACTTCGTATA ATGTATGC TATACGAAGTTAT-3' SPACER REGION; (b) not undergoing specific DNA recombination with the wild type loxP sequence even in the presence of a recombinase Cre; and (c) undergoing specific DNA recombination with another variant loxP sequence having the same sequence in the presence of the recombinase Cre.