INHIBITION OF GENE EXPRESSION

摘要

There is provided a method of inhibiting gene expression by locating a 5′-5 donor splicing site sequence in the 3′ UTR of a gene or within coding sequence containing the stop codon of a gene. Stability of homologous mRNA in trans is also adversely affected leading to a reduction in expression. A polynucleotide containing a 5′-donor splicing site sequence in either coding sequence containing the stop codon or the 3′ UTR is also provided and in one embodiment is in the form of a vector. The 5′-donor splicing site sequence can be present in multiple copies, for example as a tandem repeat. In one embodiment the 5-donor splicing site sequence has the sequence 5′-MAGGTRAGTA-3′ where M is A or C and R is A or G.