发明名称 METHOD FOR PRODUCING beta-PHOSPHOGLUCOMUTASE, METHOD FOR PRODUCING THE SAME AND USE THEREOF
摘要 <P>PROBLEM TO BE SOLVED: To provide a &beta;-phosphoglucomutase having excellent heat resistance, a method for producing the same and uses of the same. <P>SOLUTION: It has been found that the &beta;-phosphoglucomutase recombinase derived from Thermoanaerobacter brockii ATCC 35047 known as an anaerobic thermophilic bacterium having excellent heat resistance has excellent heat resistance to enable enzymatic reaction even at 65&deg;C, by measuring properties of the enzyme in purified state. The enzyme can be used at a temperature higher than the use temperature of conventional enzyme and, accordingly, reaction for a long time period can be performed with a small amount of the enzyme and risk of sundry germ contamination of the reaction liquid is reduced. There are further provided a DNA encoding the enzyme, a method for producing the enzyme, and a method for producing an oligosaccharide by using the enzyme. <P>COPYRIGHT: (C)2010,JPO&INPIT
申请公布号 JP2010148502(A) 申请公布日期 2010.07.08
申请号 JP20090268783 申请日期 2009.11.26
申请人 HAYASHIBARA BIOCHEM LAB INC 发明人 OKAMOTO IWAO;AGA SO;CHAEN HIROTO;FUKUDA SHIGEHARU
分类号 C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/12;C12P19/00 主分类号 C12N15/09
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