METHOD FOR PRODUCING APXIIA PROTEIN WHICH IS DISPLAYED ON YEAST CELL SURFACE AND USE THEREOF AS FEEDSTUFF ADDITIVE

摘要

PURPOSE: A method for preparing ApxIIA protein which is displayed on the surface of yeast is provided to maintain protein structure and to develop effective oral vaccine. CONSTITUTION: A method for displaying ApxIIA protein on cell surface comprises a step of transforming yeast with a recombinant vector containing sequence encoding ApxIIA protein and cell wall adhesion sequence. A signal peptide is amylase 1A signal peptide(ASP). The cell wall adhesion sequence is an anchor DNA fragment containing half of 3' of alpha-aglutin gene(AGA1-C320) encoding 320 amino acids of C-terminal. The yeast is Saccharomyces cerevisiae. A feed supplement contains prepared ApxIIA protein.