A PREPARATION METHOD OF SOLUBILITY RECOMBINANT PROTEIN BY USE OF PEPTIDYL-PROLYL CIS-TRANS ISOMERASE B AS A FUSION EXPRESSION PARTNER

摘要

PURPOSE: A method for preparing a recombinant protein using peptidyl-proline cis-trans isomerase B(PpiB) as a fusion expression partner is provided to improve solubility and folding ability. CONSTITUTION: A method for preparing a recombinant protein comprises: a step of preparing an expression vector having peptidyl-proline cis-trans isomerase B(PpiB) gene and foreign protein gene; a step of introducing the expression vector to host cells to make a transformant; a step of culturing the transformant; and a step of inducing the expression of recombinant protein. The PpiB is denoted by sequence number 1. The expression vector additionally contains protein restriction enzyme recognition site.