A PREPARATION METHOD OF SOLUBILITY RECOMBINANT PROTEIN BY USE OF BACTERIOFERRITIN AS A FUSION EXPRESSION PARTNER

摘要

PURPOSE: A method for preparing a recombinant protein using bacterioferritin(Bfr) as a fusion expression vector is provided to improve solubility and folding ability and to use in producing medical and industrial protein. CONSTITUTION: A method for preparing a recombinant protein comprises: a step of preparing an expression vector having a gene of bacterioferritin and foreign protein gene; a step of introducing the expression vector to host cells to produce a transformant; and a step of culturing the transformant to induce the expression of recombinant protein. The bacterioferritin is denoted by sequence number 1. The foreign protein is antigen, antibody, cell receptor, enzyme, structural protein, serum, or cell protein. The foreign protein is granulocyte colony-stimulating factor(hGCSF).