USE AND PRODUCTION OF CITRATE-STABLE NEUTRAL METALLOPROTEASES

摘要

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease engineered to resist citrate-induced autolysis.