NUCLEIC ACID DERIVED FROM HEPATITIS C VIRUS AND EXPRESSION VECTOR, TRANSFORMED CELL, AND HEPATITIS C VIRUS PARTICLES EACH PREPARED BY USING SAME

摘要

A nucleic acid which comprises a 5'-untranslated region, an NS3 protein-encoding region, an NS4A protein-encoding region, an NS4B protein-encoding region, an NS5A protein-encoding region, an NS5B protein-encoding region and a 3'-untranslated region of a hepatitis C virus genome, wherein the NS3 protein-encoding region, the NS5A protein-encoding region or the NS5B protein-encoding region contains a nucleotide substitution that induces at least one amino acid substitution selected from a group consisting of M(1205)K, F(1548)L, C(1615)W, T(1652)N, A(2196)T, A(2218)S, H(2223)Q, Q(2281)R, K(2520)N and G(2374)S wherein the amino acid substitution is defined by employing the amino acid sequence depicted in SEQ ID NO:6 shown in the Sequence Listing as a reference sequence.