发明名称 |
EXPRESSION VECTOR FOR PRODUCING PROTEIN DERIVED FROM FOREIGN GENE IN LARGE QUANTITY USING ANIMAL CELLS, AND USE THEREOF |
摘要 |
<p>Studies have been made extensively, and such an expression vector can be successfully constructed that enables the high-level production of a protein derived from a foreign gene in a mammal host cell. The expression vector comprises a gene cassette which contains a translation-impairing dihydrofolate reductase gene cistron and a cloning site into which a foreign gene is to be integrated, wherein codons in the translation-impairing dihydrofolate reductase gene cistron are altered to those which have been used in mammal cells with lowest frequencies to reduce the expression level thereof, and the cloning site is located between a highly transcriptionally active promoter and a highly stable polyadenylation signal.</p> |
申请公布号 |
CA2748011(A1) |
申请公布日期 |
2010.07.01 |
申请号 |
CA20092748011 |
申请日期 |
2009.12.22 |
申请人 |
NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY;FUSO PHARMACEUTICAL INDUSTRIES, LTD. |
发明人 |
TAHARA, HIROSHI;SUZUKI, YUSUKE;YAMAMOTO, KEIICHI;KITAHARA, YUZURU;SUZUKI, YASUHIKO |
分类号 |
C12N15/09;C12P21/02;C12Q1/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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