ALTERNATIVELY-SPLICED ISOFORM OF MU-OPIOID RECEPTOR GENE WITH CELL EXCITATORY FUNCTION

摘要

Splice variant forms of human OPRM1 and polynucleotide sequences encoding the OPRM1 splice variants are provided, including the novel sixtrans- membrane isoforms encoded by MOR-1 K1 and MOR-1 K2. Also provided are methods of screening compounds for their ability to regulate the activity of MOR-1 splice variants and of producing antibodies to the novel isoforms. Recombinant vectors, cells, and transgenic animals comprising the novel polypeptides or polynucleotides are described. Methods and assay kits for detecting the presence of a six transmembrane µ-opioid receptor isoform polypeptide are also provided.