ENHANCED ON-CHIP SERS BASED BIOMOLECULAR DETECTION USING ELECTROKINETICALLY ACTIVE MICROWELLS
摘要
A method for detecting target nucleic acids such as SNPs is provided. The method comprises performing a ligase detection reaction (LDR), performing surface enhanced Raman scattering (SERS) on the LDR, and analyzing the outcome of the LDR using analysis and/or quantification of the SERS by detecting an emitted Raman signature. The LDR-SERS method can be used for sensitive and specific detection of any nucleic acid sequence of interest. A microfluidic SERS detection device is also provided. The device comprises electrokinetically active microwells for mixing and concentrating analytes and in which analytes can be quantified. The device can be used for performing the LDR-SERS method in optofluidic chip format.
申请公布号
WO2010045357(A3)
申请公布日期
2010.07.01
申请号
WO2009US60675
申请日期
2009.10.14
申请人
CORNELL UNIVERSITY;ERICKSON, DAVID;HUH, YUN SUK;BATT, CARL A.;LOWE, ADAM JOSEPH
发明人
ERICKSON, DAVID;HUH, YUN SUK;BATT, CARL A.;LOWE, ADAM JOSEPH