<p>A blood supply device is provided with a pulsation pump (10) for intermittently sending out blood by pulsation, a liquid containing section (20) for temporarily containing the blood sent out from the pulsation pump (10), and a check valve (23) for blocking reverse flow from the liquid containing section (20) to the pulsation pump (10), and the blood supply device is adapted to supply the blood from the pulsation pump (10) into a human body via the liquid containing section (20). The liquid containing section (20) is provided with a variable chamber (22) having an inner volume which changes due to elasticity of the variable chamber (22). A portion of the blood introduced into the liquid containing section (20) in a contraction period of the pulsation pump (10) is contained in the variable chamber (22) by expansion of the variable chamber (22), and the variable chamber (22) is elastically contracted in an expansion period of the pulsation pump (10) to discharge the contained blood. The blood supply device can always continuously supply pulsating blood flow.</p>