发明名称 |
METHOD FOR PREPARATION OF RECOMBINANT DNA |
摘要 |
<p>The problem to be solved in the present invention is to provide a simplified and efficiently improved DNA recombination method. The above problem can be solved with the present method for preparing a recombinant DNA by inserting a DNA fragment of interest into a vector DNA, the method comprising the step of carrying out the following reactions at the same reacting location at the substantially simultaneouse time: (a) a reaction for simultaneously cleaving a site of the vector for inserting the fragment and a DNA containing the fragment in the presence of a restriction enzyme whose DNA recognition site and DNA cleavage site are discrete; and (b) a reaction for inserting the fragment into the vector in the presence of a DNA ligase.</p> |
申请公布号 |
EP2202313(A1) |
申请公布日期 |
2010.06.30 |
申请号 |
EP20080792415 |
申请日期 |
2008.08.12 |
申请人 |
NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY |
发明人 |
NAGAI, TAKEHARU;KOTERA, IPPEI |
分类号 |
C12N15/66;C12N15/64 |
主分类号 |
C12N15/66 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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