STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS L3F9 - PRODUCER OF MONOCLONAL ANTIBODIES SUITABLE FOR USE IN SANDWITCH IMMUNOENZYMOMETRIC SYSTEM FORMAT FOR DETECTING VP35 PROTEIN OF MARBURG VIRUS AND 3F9 MONOCLONAL ANTIBODIES PRODUCED BY SAID STRAIN OF HYBRID CELLS

摘要

FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to biotechnology and can be used in immunodiagnosis of Marburg haemorrhagic fever. A strain of hybrid animal cells Mus musculus L. 3F9 is formed, which is deposited in the collection of cell cultures of The State Research Center of Virology and Biotechnology VECTOR. The hybridoma strain produces monoclonal antibodies which are specific to the VP35 protein of the Marburg virus (Popp strain) (hereinafter MCA). MCA 3F9 produced by hybrid animal cells Mus musculus L. 3F9 relate to a subclass of immunoglobulins IgGI, having a heavy 55 kDa and a light 25 kDa chain and having a unique feature of detecting the VP35 protein of the Marburg virus (Popp strain) in a "sandwich" immunoenzymometric system format owing to antigen "capture" properties and simultaneously be an indicator, labeled biotin. The antigen epitope for MCA 3F9 produced by the 3F9 hybridoma is localised between 252 and 278 aminoacid residues. ^ EFFECT: invention enables to obtain MCA with specificity to VP35 protein of the Marburg virus (Popp strain), suitable for immunodiagnosis of Marburg haemorrhagic fever. ^ 2 cl, 3 dwg, 1 tbl, 5 ex