A NANOPARTICLE FOR SEPARATING PROTEIN, METHOD FOR PREPARING THE SAME, AND METHOD FOR SEPARATING AND PURIFYING PROTEIN USING THE SAME

摘要

PURPOSE: A nanoparticle for isolating proteins is provided to isolate and purify GST-tagged protein using a magnetic nanoparticle on which glutathione is conjugated. CONSTITUTION: A nanoparticle for isolating GST-tagged protein has a magnetic nanoparticle surface on which glutathione group is conjugated. The magnetic nanoparticle is conjugated with a silica layer. The glutathione group is conjugated with 2-pyridyl disulfide which is bound to the silica layer. A method for manufacturing the nanoparticle for isolating the GST tagged protein comprises: a step of coating the surface of magnetic nanoparticle with the silica layer; a step of binding 2-pyridyl disulfide group to the silica layer; and a step of binding glutathione group to 2-pyridyldisulfide group.