| 摘要 |
<p>A rapid method for diagnosing tuberculosis (TB) in a subject is described. Antibodies are used to detect the presence of specific biomarkers, such as cytokine or chemokines, and in particular IFN-gamma or IP-10, in an unstimulated sample from the subject. An indicator is used to indicate binding of the antibodies to the biomarkers, indicating that the patient has active extrapulmonary tuberculosis. The method can be performed using a test strip containing the antibodies and indicator and onto which the sample is placed, using an ELISA assay, or using flow cytometry, a bio-analyser, a waveguide biosensor or surface enhanced Raman spectroscopy. Results can be available within a few minutes to a few hours, depending on the type of test device used. The method can detect extrapulmonary tuberculosis and can distinguish between active and latent tuberculosis. A kit including antibodies and a chromatographic indicator and instructions for use is also described.</p> |
