发明名称 |
Peptide Standards |
摘要 |
The invention relates to methods for making a peptide standard for mass spectrometry said method comprising (a) identifying endopeptidase cleavage sites in a parent polypeptide sequence of interest; (b) selecting peptide sequences from said parent polypeptide which are defined by endopeptidase cleavage sites of step (a); (c) adding a C-terminal extension to each selected sequence; wherein if the endopeptidase cleavage site is C-terminal to its recognition sequence then the C-terminal extension comprises 1 to 6 amino acids, wherein if the endopeptidase cleavage site is N-terminal to its recognition sequence then the C-terminal extension comprises said recognition sequence, wherein if the endopeptidase cleavage site is within its recognition sequence then the C-terminal extension comprises the remainder of said recognition sequence C-terminal to the cleavage site; and (d) synthesising a peptide having the extended amino acid sequence of step (c). The invention also relates to peptide standards and methods of analysing samples.
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申请公布号 |
US2010159473(A1) |
申请公布日期 |
2010.06.24 |
申请号 |
US20080597872 |
申请日期 |
2008.05.02 |
申请人 |
KING'S COLLEGE LONDON;GUY'S AND ST. THOMAS' NHS FOUNDATION TRUST |
发明人 |
TURNER CHARLES;DALTON RAYMOND NEIL |
分类号 |
G01N33/53;C07K1/107;C07K14/00 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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