摘要 |
Described herein are improved methods of pretreating lignocellulosic biomass. One aspect of the invention relates to a two-stage pretreatment process comprising a relatively low severity steam treatment, a controlled pH pretreatment, or autohydrolysis, followed by hydrolysis with dilute acid or hot water at a relatively low temperature. In certain embodiments, the methods increase hemicellulose sugar yields, substrate digestibility, and suitability for fermentation as compared to steam explosion or acid hydrolysis alone. The two-stage pretreatment processes also employ few chemicals, minimizing the costs associated with pretreatment of lignocellulosic biomass. Moreover, the two-stage pretreatment process may expand the range of suitable feedstocks for bioethanol production. |