Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display

摘要

The present invention is directed to recombinant nucleic acid constructs comprising nucleic acid sequences encoding a Pichia pastoris PIR secretion signal peptide for secretion or an anchor domain peptide for surface display of a heterologous polypeptide. The present invention is further directed to methods for obtaining a heterologous polypeptide secreted into the culture medium or displayed on the cell surface. The present invention is also directed to transformed cells containing the recombinant nucleic acid constructs.