发明名称 |
CELL-SPECIFIC REPLICATION-COMPETENT VECTOR |
摘要 |
The present invention provides a method for constructing a vector which expresses and replicates a gene specifically to particular cells; a DNA having a gene expression regulatory activity used for its construction; and further, a treatment method comprising the steps of introducing said vector into particular living tissues or cells such as malignant tumors and the like, and said vector expressing and replicating a gene. A cell-specific replication-competent vector which does not act to normal cells in an adult body is constructed by obtaining a transcriptional initiation regulatory region of human calponin gene which is expressed specifically in cells; by linking it upstream of a viral replication-related gene; and by integrating it into a virus DNA. Said constructed cell-specific replication-competent vector is introduced into malignant tumor cells to selectively injure tumor cells or proliferating smooth muscle cells of tumor neovascular.
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申请公布号 |
CA2447300(C) |
申请公布日期 |
2010.06.22 |
申请号 |
CA20022447300 |
申请日期 |
2002.04.30 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
TAKAHASHI, KATSUHITO;YAMAMURA, HISAKO;MIYATAKE, SHIN-ICHI |
分类号 |
C12N15/09;C12N15/11;A61K35/76;A61K48/00;A61P35/00;C12N5/10;C12N15/861;C12N15/869 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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