Sensors for biomolecular detection and cell classification

摘要

A sensor device is provided for detecting an analyte in a sample in which an analyte is bound to a detection reagent to form a bound complex. The device comprises (a) a sample (5) comprising an ionic analyte and a detection reagent in a conductive fluid, wherein the detection reagent has a net charge different from the analyte; (b) a first permeable polymeric hydrogel plate (3) and a first spacer plate (8), which plates provide a compartment for the sample; (c) an anode (1) juxtaposed to the outside of the first hydrogel plate and not in contact with the sample; (d) a cathode (9) juxtaposed to the outside of the first spacer plate and not in contact with the sample; (e) a voltage generator (10) to apply an electric potential to the anode and cathode; and (f) a detector (11). The bound complex formed from the analyte and detection reagent is detected by the detector because the bound complex has a charge that causes it to migrate in a direction opposite from that of the unbound analyte when the electric potential is applied.