RECOMBINANT NUCLEIC ACID SEQUENCES AND METHODS FOR DETERMINING BOTH GENOTOXICITY AND MUTAGENICITY OF A SAMPLE AND THE KINETICS OF THE GENOTOXICITY

摘要

A recombinant nucleic acid sequence comprising an SOS regulated promoter with an induction ratio higher than 40, said promoter being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter resulting in a signal that can be assayed as light production. A biosensor comprising such a nucleic acid sequence and methods for determining genotoxicity and the presence of multiple genotoxic compounds using such a biosensor are disclosed.