| 摘要 |
An RNA polymerase comprising a wild type RNA polymerase with at least one amino acid modified so as to have a higher ability of incorporating 3'-deoxyribonucleotide or a derivative thereof than that of the corresponding wild type RNA polymerase. Specifically, for example, an RNA polymerase comprising a wild type RNA polymerase wherein at least one amino acid present in the nucleotide bonding site, for example, phenylalanine, is substituted by tyrosine. The RNA polymerase has little or no bias of incorporation between ribonucleotide and 3'-deoxyribonucleotide, between ribonucleotides having different bases, and between deoxyribonucleotides having different bases.
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