METHOD OF HIGH-POLYMERIC RNA MANUFACTURE FROM YEAST

摘要

FIELD: medicine. ^ SUBSTANCE: method of high-polymeric RNA manufacture from yeast includes yeast slurring in 1-2% aqueous solution of oleic acid titrated with alkali up to pH 7-8 followed by suspension's long simmering. Hot lysate is precipitated for 22 hours at room temperature. High-polymeric RNA is precipitated from supernatant with sodium chloride; extracted cake, containing high-polymeric RNA is flushed subsequently with 2 doses of 3 M solution of sodium chloride and 5 doses of 94% ethanol by resuspending at room temperature followed by low-speed centrifugation. ^ EFFECT: possibility to obtain target product - high-polymeric RNA. ^ 1 ex