发明名称 |
METHOD FOR PURIFICATION OF RECOMBINANT INTERFERON -1b |
摘要 |
FIELD: medicine. ^ SUBSTANCE: proposed method of purification of human recombinant interferon beta-1b (rIFN beta-1b) provides for optimisation of processes of purification of inclusion bodies, their dissolution and refolding of target protein Purification of human rIFN beta-1b is conducted with zwittehent 3-14 as a main detergent for purification and dissolving of inclusion body of Ecoli with the following refolding of rIFN beta-1b. After that prechromatographic processing of a sample is conducted and after that chromatographic processing of Ceramic S and of Source S30 and oxidation using a mixture of cysteamine/cystamine are performed. Then gel-filtration on Superdex 75 Prep grade, chromatographic processing on Source Q30, gel-filtration on Sephacril S200HR are made and finally the effectiveness of purification of the target protein by high performance liquid chromatography is monitored. ^ EFFECT: raise of the target protein yields and obtaining human rIFN beta-1b which meets the requirements of normative documents with high levels of specific antiviral activity. ^ 5 tbl, 3 ex |
申请公布号 |
RU2392282(C1) |
申请公布日期 |
2010.06.20 |
申请号 |
RU20080139497 |
申请日期 |
2008.10.06 |
申请人 |
UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT BIOORGANICHESKOJ KHIMII IM. AKADEMIKOV M.M. SHEMJAKINA I JU.A. OVCHINNIKOVA RAN;ROSSIJSKAJA FEDERATSIJA, OT IMENI KOTOROJ VYSTUPAET FEDERAL'NOE AGENTSTVO PO NAUKE I INNOVATSIJAM |
发明人 |
VOROB'EV IVAN IVANOVICH;PONOMARENKO NATAL'JA ALEKSANDROVNA;MIROSHNIKOV ANATOLIJ IVANOVICH;SMIRNOV IVAN VITAL'EVICH |
分类号 |
A61K38/21;C07K1/18;C07K1/36;C07K14/565 |
主分类号 |
A61K38/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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