STRAIN OF STREPTOALLOTEICHUS CREMEUS SUBSP TOBRAMYCINI VKPM S-1084- PRODUCER OF APRAMICYNE AND METHOD OF APRAMICYNE OBTAINING

摘要

FIELD: medicine. ^ SUBSTANCE: method involves cultivation of strain Streptoalloteichus cremeus subsp. tobramycini VKPM S-1084, producer of apramicyne. Emanation of apramicyne from culture fluid with sorption on carboxylic cationites followed by chromatographic purification with two-column method by stepwise elution with solutions (0.19-0.22)n of ammonia. The resulting eluate is concentrated in vacuum and precipitated with excess of acetone apramicyne in the form of sulfate. ^ EFFECT: increased output of apramicyne. ^ 2 cl, 1 tbl, 2 ex