| 摘要 |
The present invention is related to the production, partition, recovery and/or purification of biological and non-biological compounds by means of aqueous two-phase systems implemented in non-rigid geometry containers, preferably bags for blood/plasma. The aqueous two-phase system has been widely characterised in that it uses only two rigid containers to be constructed. In the present invention, the aqueous two-phase system (ATPS) is formed in non-rigid geometry containers, particularly blood/plasma bags, where the formation of the ATPS in non-rigid containers allows biological and non-biological compounds to be produced (optionally by extractive conversion and/or extractive fermentation), recovered and purified. The samples to be processed may be of different nature, including all types of biological fluids, process effluents or artificially-generated mixtures. The ATPS is generated by mixing in situ or ex situ the compounds forming the same in the suitable amounts based on the selec ted parameters. The system is agitated and left aside to achieve the separation of phases and balance thereof. The phase where the pollutants are partitioned is removed from the device, while the phase containing the interest compound may rest in the container, where the phase may be stored under ideal conditions to be preserved, or recovered to be processed in subsequent stages.
|
